High surface cultivation system with surface increasing...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/16 (2006.01) C12M 3/04 (2006.01)

Patent

CA 2677721

The present invention is directed culture vessel suitable for cultivation of cells and/or tissues comprising at least one reversibly clo sable aperture in the vessel wall, and at least one surface-increasing substrate within the vessel, said substrate being made of a single mold. The invention further provides a system comprising at least two vessels being interconnected via at least one aperture in their vessel wall, and a cultivation process using such a vessel or system, in which at least one type of cells, tissue, tissue-like cell cultures, organs, organ- like cell cultures, or multicellular organisms are cultivated in the presence of at least one fluid or solid medium necessary for growing and/or cultivating the aforesaid culture.

La présente invention porte sur un récipient de culture approprié pour la culture de cellules et/ou de tissus, comprenant au moins une ouverture apte à être fermée de façon réversible dans la paroi de récipient, et au moins un substrat d'augmentation de surface à l'intérieur du récipient, ledit substrat étant fait d'un simple moule. L'invention concerne en outre un système comprenant au moins deux récipients qui sont interconnectés par l'intermédiaire d'au moins une ouverture dans leur paroi de récipient, et un procédé de culture utilisant un tel récipient ou un tel système, dans lequel au moins un type de cellules, tissu, cultures de cellules de type tissus, organes, cultures de cellules de type organes ou organismes multicellulaires et cultivé en présence d'au moins un milieu fluide ou solide nécessaire pour développer et/ou cultiver la culture mentionnée ci-dessus.

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