High temperature and alkaline stable catalase

Details High temperature and alkaline stable catalase High temperature and alkaline stable catalase

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C12N 9/08 (2006.01) C12N 11/10 (2006.01) C12N 11/12 (2006.01) C12N 15/63 (2006.01) C12P 3/00 (2006.01)

Patent

CA 2543442

The invention relates to thermal and pH stable catalases. One catalase of the invention was purified and characterized from Thermus brockianus. As a part of the characterization, the enzyme was compared to typical catalases from commercial sources and found to be significantly more thermal/alkaline stable than these other enzymes. The catalase purified from T. brockianus consists of four identical subunits having a molecular mass of approximately 42.5 kDa, for a total molecular mass of approximately 178 kDa.

La présente invention concerne des catalases stables à la température et au pH. L'une des catalases de l'invention a été purifiée et caractérisée à partie de Thermus brockianus. Pour la caractériser, on a notamment comparé l'enzyme à des catalases typiques du commerce, et elle s'est avérée notoirement plus stable à la chaleur et au milieu alcalin que ces autres enzymes. La catalase purifiée à partir de T. brockianus est constituée de quatre sous-unités identiques d'une masse moléculaire d'environ 42,5 kDa pour une masse moléculaire totale d'environ 178 kDa.

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