High temperature cryogenic preservation of biologically...

A - Human Necessities – 01 – N

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A01N 1/02 (2006.01)

Patent

CA 2410896

Viable biological material is cryogenically preserved (cryopreservation) by preparing the material for freezing, immersing the material in a tank of cooling fluid, and circulating the cooling fluid past the material at a substantially constant predetermined velocity and temperature to freeze the material. A method according to the present invention freezes the biologic material quickly enough to avoid the formation of ice crystals within cell structures (vitrification). The temperature of the cooling fluid is preferably between -20 ~C and -30 ~C, which is warm enough to minimize the formation of stress fractures in cell membranes due to thermal changes. Cells frozen using a method according to the present invention have been shown to have approximately an 80 percent survival rate, which is significantly higher than other cryopreservation methods.

La conservation cryogénique (cryoconservation) de matières biologiques viables, consiste à préparer la matière à congeler, à immerger cette matière dans un réservoir de fluide réfrigérant et en faisant circuler ce fluide réfrigérant autour de la matière à une vitesse et à une température prédéterminées, sensiblement constantes, afin de congeler la matière. Le présent procédé permet de congeler la matière biologique assez rapidement pour éviter la formation de cristaux de glace dans les structures cellulaires (vitrification). La température du fluide de refroidissement est de préférence comprise entre 20· et 30 ·C, ce qui constitue une température assez élevée pour réduire l'apparition de fractures de contrainte dues aux changements de température dans les membranes cellulaires. Les cellules congelées par le procédé décrit ont montré un taux de survie de 80 pour-cent environ, ce qui est largement supérieur aux autres procédés de cryoconservation.

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