High temperature reverse transcriptases

Details High temperature reverse transcriptases High temperature reverse transcriptases

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 9/12 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2071213

Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 94 kDa TaQ, 62 kDa- Taq, nTth and recombinant Tth DNA polymerase. Reverse Transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.

Procédés de réplication et d'amplification de séquences d'ARN à l'aide de polymérases d'ADN thermoactives. La transcription inverse d'ARN est catalysée par, par exemple, la polymérase d'ADN de Taq de 94 kDa, de Taq de 62 kDa, de nTth et de Tth recombiné. La transcription inverse est couplée à l'amplification par réaction en chaîne de polymérases (RCP) dans une procédure mettant en oeuvre une seule enzyme, utilisant une polymérase thermostable.

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