High temperature reverse transcription using mutant dna...

C - Chemistry – Metallurgy – 12 – P

Patent

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C12P 19/34 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2342277

The present invention relates to improved reverse transcription methods using a modified thermostable DNA polymerases, particularly in a magnesium ion buffer. These methods are particularly useful in combined reverse-transcription/amplification reactions.

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