High throughput assay for cancer cell growth inhibition

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12M 1/34 (2006.01)

Patent

CA 2593494

A high-throughput, anchorage-independent assay is described, which screens compounds for inhibition of cancer cell growth. The assay utilizes a three- dimensional matrix or semi-solid media transfected with the subject compound, and enables live colony growth determination and imaging.

La présente invention a trait à un dosage à haut débit indépendant d'ancrage qui réalise le criblage de composés pour l'inhibition de la croissance de cellules cancéreuses. Le dosage utilise une matrice tridimensionnelle ou un support semi-solide transfecté avec le composé de l'invention, et permet la détermination et l'imagerie en temps réel de la croissance de colonies.

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