High throughput assay system

G - Physics – 01 – N

Patent

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G01N 33/487 (2006.01) C12M 1/34 (2006.01)

Patent

CA 2502238

A device and a method that permits rapid application of experimental solutions to both (i.e. intracellular and extracellular) surfaces of a membrane patch is described. In one embodiment, this is accomplished by mounting a membrane patch on a hole through the side of a hollow tube such that one surface can be readily perfused on the outside of the tube while simultaneously perfusing the inside of the tube. Thus, by measuring changes in membrane traffic using any of a variety of means known in the art, it is possible to determine the effect of test compounds presented to the intracellular and/or extracellular surface on membrane traffic. As can be seen, the instant device and method have the advantage of allowing both sides of the membrane to be accessed simultaneously, as described below. This is in contrast to existing patch clamp techniques where only a single membrane surface is readily accessible

L'invention concerne un dispositif et un procédé permettant l'application rapide de solutions expérimentales aux deux surfaces (c'est-à-dire intracellulaire et extracellulaire) d'un lambeau de membrane. Dans un mode de réalisation, ceci est obtenu par montage d'un lambeau de membrane sur un trou à travers le côté d'un tube creux de telle manière qu'une surface peut être mise facilement sous perfusion à l'extérieur du tube tout en mettant simultanément sous perfusion l'intérieur du tube. Ainsi, par mesure des changements dans le trafic membranaire, à l'aide de n'importe lequel d'une variété de moyens connus dans l'art, il est possible de déterminer l'effet de composés d'essai présentés à la surface intracellulaire et/ou extracellulaire sur le trafic membranaire. Tel qu'on peut le voir, le dispositif et le procédé de l'invention présentent l'avantage de permettre l'accès simultanément aux deux cotés de la membrane, tel que le décrit l'invention. Ce procédé se distingue des techniques de patch clamp existantes dans lesquelles une seule surface membranaire est accessible facilement.

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