High throughput cellular response assay using microarrays

Details High throughput cellular response assay using microarrays High throughput cellular response assay using microarrays

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01) B01J 19/00 (2006.01) C12Q 1/68 (2006.01) G01N 21/64 (2006.01) G01N 33/50 (2006.01) G01N 33/551 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2487929

The present invention relates to methods for screening of cellular responses of cellular components comprising:(a) providing cellular components on the surface of a substrate, said substrate having immobilized thereon an array of detector molecules;(b) delivering test compounds to positions on the substrate corresponding to the arrayed detector molecules on the surface of said solid substrate;(c) incubating said test compounds with said cellular components on the surface of the solid support, under conditions allowing the induction of cellular responses;(d) assaying said cellular responses; and,identifying and characterizing the cellular responses induced by said test compounds. The present invention further relates to the uses of said methods as well as microarrays and kits for carrying out said methods.

La présente invention concerne des méthodes destinées au criblage des réponses cellulaires de composants cellulaires, consistant (a) à placer des composants cellulaires sur la surface d'un substrat sur lequel est immobilisé un réseau de molécules de détection, (b) à placer des mélanges de contrôle sur le substrat, au niveau des positions des molécules de détection en réseau sur la surface dudit substrat solide, (c) à incuber les mélanges de contrôle et lesdits composants cellulaires situés sur la surface du support solide dans des conditions permettant l'induction de réponses cellulaires, (d) à analyser les réponses cellulaires, et à identifier et caractériser les réponses cellulaires induites par lesdits mélanges de contrôle. La présente invention concerne également des applications de ces méthodes, ainsi que des microréseaux et des nécessaires destinés à la mise en oeuvre de ces méthodes.

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