C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/20 (2006.01) C12N 9/00 (2006.01)
Patent
CA 2465543
A method for high throughput micro-purification of a library of tag-free biological compounds is claimed wherein a population of discrete liquid samples comprising the library of tag-free biological compounds is contacted with a solid chromatographic material comprising a functional group selected from ion exchange materials, hydrophobic materials, affinity materials and hydrophobic charge induction materials so as to retain the tag-free biological compounds of the library, isolating the chromatographic material retaining the biological compounds, releasing the biological compounds of the library from the solid chromatographic material and collecting the released biological compounds of the library to produce a population of samples comprising the isolated biological compound.
L'invention concerne un procédé de micro-purification à débit élevé d'une bibliothèque de composés biologiques exempts de marqueurs. Selon ledit procédé, une population d'échantillons liquides discrets contenant la bibliothèque de composés biologiques exempts de marqueurs est mise en contact avec un système de chromatographie solide composé d'un groupe fonctionnel choisi parmi des substances d'échange ionique, des substances hydrophobes, des substances d'affinité et des substances d'induction de charge hydrophobe, de manière à retenir les composés biologiques exempts de marqueurs de la bibliothèque. Ensuite, le système de chromatographie retenant les composés biologiques est isolé, les composés biologiques de la bibliothèque sont extraits du système de chromatographie, et les composés biologiques de la bibliothèque extraits sont recueillis de manière à former une population d'échantillons contenant le composé biologique isolé.
Christensen Lars Lehmann Hylling
Frisner Henrik
Novozymes A/s
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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