High throughput mass spectrometry

Details High throughput mass spectrometry High throughput mass spectrometry

C - Chemistry, Metallurgy

40

B

C40B 30/10 (2006.01) C12M 1/34 (2006.01) C12N 1/00 (2006.01) C12N 9/14 (2006.01) C12N 15/09 (2006.01) C12Q 1/02 (2006.01) C12Q 1/25 (2006.01) C12Q 1/68 (2006.01) C40B 30/00 (2006.01) C40B 30/06 (2006.01) G01N 27/62 (2006.01) G01N 30/72 (2006.01) G01N 33/483 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01) G01N 37/00 (2006.01)

Patent

CA 2361384

Apparatus and methods for high throughput mass spectrometry are provided. The methods involve sample preparation in an off-line parallel purification system. Such methods include but are not limited to the use of an appropriate buffer when generating samples or the use of a solid support for tagged components. The samples prepared in this way do not then need to be column separated. The apparatus provided includes a cell growth plate for growing cells and generating products and/or reactants, an off-line parallel purification system, a mass spectrometer, and an automatic sampler that transports samples and injects them into the mass spectrometer of the apparatus. The methods and apparatus described are used, for example, in screening enzyme reaction pathways.

L'invention concerne un appareil et des procédés permettant de réaliser une spectrométrie de masse à haut rendement. Ces procédés consistent à préparer des consistent, entre autres, à utiliser un tampon approprié pendant la génération d'échantillons ou à utiliser un support solide pour des composants marqués. Les échantillons ainsi préparés n'exigent pas une séparation en colonnes. Cet appareil comporte une plaque de culture cellulaire servant à cultiver des cellules et à obtenir des produits et/ou des réactifs, un système de purification parallèle hors circuit, un spectromètre de masse et un échantillonneur automatique qui transporte les échantillons et les injecte dans le spectromètre de masse. Ces procédés et cet appareil servent, par exemple, à cribler des voies de réaction enzymatique.

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