High throughput screen

G - Physics – 01 – N

Patent

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Details

G01N 33/543 (2006.01) G01N 27/327 (2006.01)

Patent

CA 2334770

The present invention relates to a structure comprising a biological membrane and a porous or perforated substrate, a biological membrane, a substrate, a high throughput screen, methods for production of the structure membrane and substrate, and a method for screening a large number of test compounds in a short period. More particularly it relates to a structure comprising a biological membrane adhered to a porous or perforated substrate, a biological membrane capable of adhering with high resistance seals to a substrate such as perforated glass and the ability to form sheets having predominantly an ion channel or transporter of interest, a high throughput screen for determining the effect of test compounds on ion channel or transporter activity, methods for manufacture of the structure, membrane and substrate, and a method for monitoring ion channel or transporter activity in a membrane.

La présente invention concerne une structure comportant une membrane biologique et un substrat poreux ou perforé, une membrane biologique, un dispositif de criblage à grande capacité, des procédés de production de la membrane de structure et du substrat, ainsi qu'un procédé rapide de criblage d'un grand nombre de composés d'analyses. Plus particulièrement, cette invention concerne une structure comprenant une membrane biologique adhérant à un substrat poreux ou perforé, une membrane biologique pouvant adhérer, en formant des jointures d'étanchéité très résistantes, à un substrat formé par du verre perforé, et former des feuilles constituées essentiellement par canal ionique ou un transporteur d'ions souhaité, un dispositif de criblage à grande capacité permettant de déterminer l'effet des composés d'analyses sur l'activité du canal ionique ou du transporteur, des procédés de fabrication de la structure, de la membrane et du substrat, ainsi qu'un procédé de surveillance de l'activité du canal ionique ou du transporteur d'ions dans une membrane.

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