G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/566 (2006.01) G01N 33/50 (2006.01) G01N 33/543 (2006.01) G01N 33/554 (2006.01)
Patent
CA 2336929
The present invention is related to a diagnostic and/or dosage method of an agonist and/or an antagonist and/or a modulator for a calcium-coupled receptor and/or channel and/or any other calcium-coupled protein, comprising the following successive steps: disposing the agonist and/or the antagonist and/or the modulator upon a solid support; incubating one or more cells) expressing apoaequorin and said calcium-coupled receptor with coelenterazine in order to reconstitute an active aequorin in said cell(s); adding to said solid support one or more of said cells; and obtaining the measurement of an emitted light by said cell(s). The present invention is also related to the diagnostic and/or dosage device intended for the method according to the invention.
L'invention porte sur un procédé de diagnostic et/ou de dosage d'un agoniste et/ou antagoniste et/ou modulateur d'un récepteur à couplage calcium et/ou d'un canal et/ou de toute autre protéine à couplage calcium, comportant les étapes suivantes: pose de l'agoniste et/ou de l'antagoniste et/ou du modulateur sur un support solide; incubation d'une ou plusieurs cellules exprimant l'apoaequorine et ledit récepteur à couplage calcium, avec de la coélentérazine, de manière à reconstituer une aequorine active dans la ou lesdites cellules; adjonction au support solide d'une ou plusieurs de ces cellules, et obtention d'une mesure de la lumière émise par la ou lesdites cellules. L'invention porte également sur le dispositif de diagnostic et/ou de dosage utilisé pour la mise en oeuvre du procédé de l'invention.
Detheux Michel
Dupriez Vincent
Parmentier Marc
Euroscreen S.a.
Gowling Lafleur Henderson Llp
Perkinelmer Cellular Sciences Belguim
LandOfFree
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