High-throughput screening method and apparatus

Details High-throughput screening method and apparatus High-throughput screening method and apparatus

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) C12M 1/34 (2006.01) G01N 27/06 (2006.01) G01N 33/487 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2258279

High-throughput screening method and apparatus are described. The method includes placing cells on a substrate defining a plurality of discrete microwells, at a well density of greater than about 100/cm2, with the number of cells in each well being less than about 1000, and where the cells in each well have been exposed to a selected agent. The change in conductance in each well is determined by applying a low-voltage, AC signal across a pair of electrodes placed in that well, and synchronously measuring the conductance across the electrodes, to monitor the level of growth or metabolic activity of cells contained in each well. Also disclosed is an apparatus for carrying out the screening method.

L'invention concerne un procédé et un dispositif de criblage à haut rendement. Le procédé implique de placer des cellules sur un substrat, en définissant plusieurs micropuits distincts, à une densité par puits dépassant 100/cm?2¿ environ, et un nombre de cellules par puits inférieur à environ 1000, les cellules de chaque puits ayantexposées à un agent sélectionné. Le changement de la conductance dans chacun des puits est déterminé par l'application d'un signal à basse tension par courant alternatif à travers deux électrodes placées dans le puits et la mesure simultanée de la conductance à travers les électrodes, qui permettent de surveiller le taux de croissance ou d'activité métabolique des cellules contenues dans chaque puits. Un dispositif permettant de mettre en oeuvre le procédé de criblage est également décrit dans l'invention.

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