High-throughput screening of gene function using libraries...

C - Chemistry – Metallurgy – 40 – B

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C40B 40/02 (2006.01) C12N 15/10 (2006.01) C12N 15/861 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) C40B 30/06 (2006.01) C40B 40/06 (2006.01) C40B 50/06 (2006.01)

Patent

CA 2301403

Novel means and methods for their use are provided to determine the function of the product(s) of one or more sample nucleic acids. The sample nucleic acids are synthetic oligonucleotides, DNA, or cDNA and encode polypeptides, antisense nucleic acids or GSEs. The sample nucleic acids are expressed in a host by a vehicle to alter at least one phenotype of the host. The altered phenotype(s) is identified as a means to assign a biological function to the product(s) encoded by the sample nucleic acid(s).

La présente invention concerne de nouveaux moyens et procédés destinés à déterminer la fonction du ou des produits d'un ou de plusieurs acides nucléiques échantillons. Ces acides nucléiques échantillons sont des oligonucléotides synthétiques, un ADN, ou un ADNc et codent pour des polypeptides, des acides nucléiques antisens ou pour des éléments suppresseurs génomiques (GSE). Les acides nucléiques échantillons sont exprimés dans un hôte à l'aide d'un véhicule afin d'altérer au moins un phénotype de cet hôte. Le ou les phénotypes altérés sont identifiés comme moyen d'attribuer une fonction biologique à ou aux produits codés par le ou les acides nucléiques échantillons.

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