High throughput screening of genetically modified...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12M 1/00 (2006.01)

Patent

CA 2692893

The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

Cette invention concerne un procédé et des compositions pour le criblage haut rendement d'organismes de photosynthèse génétiquement modifiés pour un état plasmique. Cette invention concerne des procédés permettant de produire une ou plusieurs protéines, y compris des enzymes de dégradation de biomasse dans une plante. Cette invention concerne également des procédés consistant à produire des voies de dégradation de biomasse dans des cellules d'algues, en particulier dans le chloroplaste. Des enzymes uniques ou plusieurs enzymes peuvent être produites selon ces modes de réalisation. Les procédés décrits dans cette invention permettent la production de biocarburant, y compris l'éthanol.

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