High throughput screening of mutagenized populations

Details High throughput screening of mutagenized populations High throughput screening of mutagenized populations

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2623539

Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

L'invention concerne des procédés efficaces d'identification à haut débit de mutations dans des gènes chez des membres de populations mutagénisées. Ces procédés consistent à isoler un ADN, regrouper, amplifier, créer des bibliothèques, séquencer à haut débit des bibliothèques, de préférence par séquençage par synthèse, identifier des mutations et identifier le membre de la population portant la mutation et identifier la mutation.

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