High throughput size-exclusive method of screening complex...

Details High throughput size-exclusive method of screening complex... High throughput size-exclusive method of screening complex...

G - Physics

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G01N 33/537 (2006.01) G01N 33/538 (2006.01) G01N 30/02 (2006.01) G01N 30/46 (2006.01) G01N 30/72 (2006.01)

Patent

CA 2362052

The invention encompasses an improved, rapid, size-exclusive method for screening complex biological materials, e.g., combinatorial libraries, natural products or samples, or mixtures of pure compounds, for small molecular weight ligands that bind specifically to a protein target, using size-exclusion separation, ultrafiltration, and mass spectrometry.

La présente invention concerne un procédé amélioré, rapide et à exclusion de taille et permettant une recherche systématique de ligands, dans des matériaux biologiques complexes, notamment dans des échantillothèques, dans des produits naturels ou des échantillons, ou des mélanges de composés purs. Les ligands considérés sont des ligands de faible masse moléculaire se liant spécifiquement à une protéine cible. La recherche se fait par séparation à exclusion de taille, ultrafiltration et spectrométrie de masse.

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