High-yield fermentation processes

Details High-yield fermentation processes High-yield fermentation processes

C - Chemistry, Metallurgy

12

N

C12N 1/00 (2006.01) C12N 1/20 (2006.01) C12N 15/31 (2006.01)

Patent

CA 2078054

-21- ABSTRACT OF THE DISCLOSURE Processes for high cell density fermination and gene over expression may utilize a culture medium containing Epsom salt which is preferably sterilized separately from nutrient sources or phosphate salts. An embodiment of the process produces divalent diarrhea vaccine antigen K88,K99 using E. coli C600 cells.

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