C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/52 (2006.01) C12P 19/26 (2006.01)
Patent
CA 2665332
The present invention relates to a method for producing sialic acid, comprising the step of culturing a microorganism in a culture medium, wherein said microorganism comprises heterologous genes encoding a sialic acid synthase (NeuB), a UDP-GlcNAc epimerase (NeuC), said micro-organism being devoid of a gene encoding CMP-Neu5Ac synthase (NeuA) or wherein a gene encoding CMP-Neu5Ac synt hase (NeuA) has been inactivated or deleted; and wherein endogenous genes coding for sialic acid aldolase (NanA), for ManNac kinase (NanK) and for sialic acid transporter (NanT) have been deleted or inactivated. It also relates to the above microorganism.
L'invention concerne un procédé de production d'acide sialique, consistant à cultiver un microorganisme dans un milieu de culture. Ledit microorganisme comprend des gènes hétérologes codant une synthase d'acide sialique (NeuB), une épimérase UDP-GlcNAc (NeuC), et il est dépourvu d'un gène codant une synthase CMP-Neu5Ac (NeuA), ou un gène codant la synthase CMP-Neu5Ac (NeuA) a été inactivé ou supprimé, des gènes endogènes codant une aldolase d'acide sialique (NanA), une kinase ManNac (NanK) et un transporteur d'acide sialique (NanT) ayant été supprimés ou inactivés. L'invention concerne également ledit microorganisme.
Centre National de La Recherche Scientifique (cnrs)
Smart & Biggar
LandOfFree
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