Highly efficient devices and methods for culturing cells

Details Highly efficient devices and methods for culturing cells Highly efficient devices and methods for culturing cells

C - Chemistry, Metallurgy

12

M

C12M 1/12 (2006.01) C12M 1/04 (2006.01) C12M 3/06 (2006.01)

Patent

CA 2671967

This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.

L'invention concerne des procédés et des dispositifs qui améliorent l'efficacité dans la culture de cellules. Ces procédés consistent à utiliser des compartiments de culture perméables au gaz qui permettent de réduire l'utilisation d'espace tout en conservant des conditions de culture uniformes, et qui sont plus adaptés à une manipulation automatique des liquides. Ils consistent à intégrer des matériaux perméables au gaz dans le format à plusieurs étages classique, pour résoudre le problème lié aux conditions de culture non uniformes. Ils comprennent des dispositifs de culture qui utilisent des surfaces constituées de silicone perméables au gaz, chargées en plasma et peuvent intégrer des surfaces de fixation classiques, telles que celles constituées de polystyrène traité par culture de tissu classique. Ces procédés concernent des dispositifs de culture qui intègrent des membranes perméables au liquide et perméables au gaz. Ce procédé permet d'obtenir davantage de bénéfices, y compris des conditions de culture optimales pendant la mise à l'échelle et une meilleure utilisation de l'espace de stockage, de l'espace de l'incubateur et de l'espace d'évacuation. De plus les risques de contamination et liés au travail sont réduits.

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