Highly-purified recombinant reverse transcriptase

Details Highly-purified recombinant reverse transcriptase Highly-purified recombinant reverse transcriptase

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2186018

A plasmid for expression of Moloney Murine Leukemia Virus-dirived reverse transcriptase in E. Coli cells deficient in the expression of indigenous RNAse activity, a method for purification of the recombinant enzyme, and a composition comprising a cloned and purified reverse transcriptase optimized for use in cDNA and nucleic acid amplification procedures.

La présente invention concerne: un plasmide permettant l'expression de la transcriptase inverse dérivée du virus de la leucémie murine de Moloney dans les cellules d'Escherichia Coli faisant preuve d'une déficience d'expression de l'activité de la ribonucléase indigène; un procédé de purification de l'enzyme de recombinaison; et une composition comprenant une transcriptase inverse clonée et purifiée, optimisée pour les procédures d'amplification d'ADNc et d'acide nucléique.

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