Highly sensitive, accurate and precise automated method and...

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G01N 15/10 (2006.01) G01N 15/14 (2006.01) G01N 33/49 (2006.01)

Patent

CA 2191829

The present invention provides a highly sensitive and accurate method and system for the discrimination and quantification of platelets in a whole blood sample using automated hematology instruments. The method and system of the invention provide the accurate measurements of platelet dry mass and platelet component concentration in both normal blood samples and in abnormal blood samples, such as those from thrombocytopenic patients. The determination of platelet dry mass and platelet component concentration can serve to assess the activation state of platelets since activated platelets possess measurably lower component concentrations and refractive indices than do unactivated platelets. The method and system of the invention also allows the clinician or skilled practitioner to determine the age of a blood sample on the basis of the measured parameter of platelet component concentration.

a présente invention comporte une méthode et un système très délicats et précis de discrimination et d'évaluation quantitative des plaquettes dans des échantillons de sang entier, au moyen d'instruments automatisés pour analyses hématologiques. Cette méthode et ce système permettent d'obtenir des mesures précises de la masse sèche plaquettaire et de la concentration en éléments plaquettaires tant dans les échantillons de sang normal que dans les échantillons de sang anormal, comme ceux provenant de patients thrombocytopéniques. La détermination de la masse sèche plaquettaire et de la concentration en éléments plaquettaires peut servir à l'évaluation de l'état d'activation des plaquettes, puisque les plaquettes activées possèdent des concentrations en éléments et des indices de réfraction sensiblement inférieurs à ceux des plaquettes non activées. La méthode et le système proposés permettent également au clinicien ou au praticien qualifié de déterminer l'âge d'un échantillon sanguin selon le paramètre mesuré de la concentration en éléments plaquettaires.

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