Hip/pap polypeptide composition for use in liver...

A - Human Necessities – 61 – K

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A61K 38/17 (2006.01) A61P 1/16 (2006.01)

Patent

CA 2529896

This invention is based on the experimental finding that HIP/PAP has mitogenic and antiapoptotic effects in vitro on hepatocytes in primary culture. Moreover, HIP/PAP is a mitogenic and anti-apoptotic molecule for hepatocytes, in vivo, during liver failure and liver regeneration. The present invention is also based on the experimental finding that HIP/PAP administration has no adverse effects in mammals. This invention concerns a pharmaceutical composition for stimulating liver regeneration in vivo including after chronic/acute liver failure, comprising an effective amount of a polypeptide comprising an amino acid sequence having 90% amino acid identity with the polypeptide consisting of the amino acid sequence starting at the amino acid residue (36) and ending at the amino acid residue (175) of sequence SEQ ID N~1, in combination with at least one physiologically acceptable excipient.

L'invention repose sur la découverture expérimentale selon laquelle la HIP/PAP exerce des effets mitogénétiques et antiapoptotiques, in vitro, sur les hépatocytes en culture primaire. Par ailleurs, la HIP/PAP est une molécule qui exerce un effet mitogénétique et antiapoptotique sur les hépatocytes, in vivo, en cas d'insuffisance hépatique ou de régénération hépatique. De plus, l'invention repose sur la découverture expérimentale selon laquelle l'administration de la HIP/PAP ne présente aucun effet secondaire chez les mammifères. L'invention concerne également une composition pharmaceutique utilisé pour stimuler la régénération hépatique, in vivo, notamment en cas d'insuffisance hépatique chronique/aiguë. Cette composition contient une quantité efficace d'un polypeptide comprenant une séquence d'amino-acides qui partage 90 % de l'identité d'amino-acides avec le polypeptide qui comporte la séquence d'amino-acides commençant par le résidu d'aminoacide (36) et se terminant par le résidu d'aminoacide (175) de la séquence SEQ ID N·1, en plus d'au moins un excipient physiologiquement acceptable.

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