Histological reconstruction and automated image analysis

G - Physics – 06 – K

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G06K 9/56 (2006.01) G06K 9/00 (2006.01) G06K 9/20 (2006.01)

Patent

CA 2366524

A method for automated image analysis (Fig. 2, 42) of a biological specimen by histological reconstruction, provided is an automated cell image method for analyzing a biological specimen that has consecutively been stained by either an in situ hybridization (ISH) method, or an immunohistochemistry (IHC) method or a nucleic acid stain, and counterstained. The method couples composite images in an automated manner for processing and analysis to identify structure in tissue that cannot be captured in a single field of view image (Fig. 2, 44) or a single staining technique, the disclosure provides a method for histological reconstruction to analyze many fields of view on potentially many slides (Fig. 2, 37) simultaneously.

L'invention concerne un procédé automatisé d'analyse d'images (Fig.2, 42) d'un échantillon biologique par reconstruction histologique. L'invention concerne également un procédé d'imagerie par cellules automatisé destiné à l'analyse d'un échantillon biologique ayant été d'abord coloré, soit par une hybridation in situ (HIS), soit par une technique d'immunohistochimie, ou bien par une coloration à base d'acide nucléique, puis contre-coloré. Ce procédé associe des images composites de manière automatisée en vue de leur traitement et de leur analyse aux fins d'identifier des structures tissulaires ne pouvant pas être capturées dans une seule prise de vue (Fig.2, 44) ou par une seule technique de coloration. L'invention concerne en outre une technique de reconstruction histologique permettant d'analyser simultanément plusieurs prises de vue sur un grand nombre potentiel de diapositives (Fig.2, 37).

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