Hiv env proteins with modifications in the v3 loop

A - Human Necessities – 61 – K

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A61K 39/21 (2006.01) C07K 14/16 (2006.01)

Patent

CA 2697370

The invention concerns modification of the V3 loop of the HIV envelope glycoprotein in order to alter its Tat--binding properties. The invention provides a mixture of (i) a HIV Tat polypeptide and (ii) a HIV Env polypeptide, wherein the Env polypeptide has one or more mutations in its V3 loop. Also a method comprising a step of: combining a HIV Env polypeptide with a HIV Tat polypeptide, wherein the Env polypeptide has one or more mutations in its V3 loop. Also a mixture of (i) a HIV Tat polypeptide and (ii) a polypeptide comprising a fragment of a V3 loop of a HIV Env polypeptide.

L'invention concerne une modification de la boucle V3 de la glycoprotéine d'enveloppe du VIH afin d'altérer ses propriétés de liaison à Tat. L'invention propose un mélange de (i) un polypeptide Tat du VIH et (ii) un polypeptide Env du VIH, le polypeptide Env ayant une ou plusieurs mutations dans sa boucle V3. L'invention porte également sur un procédé consistant : à combiner un polypeptide Env du VIH avec un polypeptide Tat du VIH, le polypeptide Env ayant une ou plusieurs mutations dans sa boucle V3. L'invention propose également un mélange de (i) un polypeptide Tat du VIH et (ii) un polypeptide comportant un fragment d'une boucle V3 d'un polypeptide Env du VIH.

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