Hivcon: an hiv immunogen and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) A61K 39/21 (2006.01) A61K 48/00 (2006.01) C07K 14/16 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01) C12P 21/02 (2006.01) C40B 40/10 (2006.01) G01N 33/554 (2006.01)

Patent

CA 2597511

The present invention provides artificial fusion proteins (AFPs) designed to elicit an anti-HIV immune response, as well as nucleic acid molecules and expression vectors encoding those proteins. The AFPs of the invention may comprise domains from various HIV proteins, such as Gag, Pol, Vif, and Env proteins, which are partial sequences. HIVCON is an AFP in which the HIV domains are from several HIV clade consensus sequences and which optionally contains additional domains which may be useful, for example, in monitoring expression levels or laboratory animal immune responses. Other aspects of the invention may include compositions and methods for inducing an anti-HIV immune response in a subject, preferably with a DNA prime-MVA boost strategy, and to induce a cell-mediated immune response.

L'invention concerne des protéines hybrides artificielles (PHA) conçues pour provoquer une réponse immunitaire anti-VIH, ainsi que des molécules d'acide nucléique et des vecteurs d'expression codant pour ces protéines. Les PHA décrites peuvent comprendre des domaines de différentes protéines VIH, telles que les protéines Gag, Pol, Vif, et Env, qui sont des séquences partielles. HIVCON est une PHA dans laquelle les domaines VIH proviennent de différentes séquences consensus d'un clade, et qui peut éventuellement contenir des domaines additionnels pouvant servir par exemple à contrôler les niveaux d'expression, ou les réponses immunitaires chez les animaux de laboratoire. D'autres aspects de l'invention peuvent comprendre des compositions et des procédés permettant de provoquer une réponse immunitaire anti-VIH chez un sujet, de préférence par une stratégie de stimulation par primo-immunisation ADN-rappels MVA, et de produire une réponse immunitaire activée par les cellules.

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