Hla d typing assay

G - Physics – 01 – N

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G01N 33/53 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1226218

- 19 - HLA D TYPING ASSAY ABSTRACT An assay for HLA D phenotype wherein antigen-pulsed monocytes are contacted with antigen-specific T lymphocytes or T cell hybridomas and the extent of selective binding between the pulsed monocytes and the T lymphocytes or T cell hybridomas determines HLA D phenotype of the monocyte. The assay is particularly useful for quickly typing donor tissue prior to transplantation.

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