C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/143, 195/67
C12N 5/00 (2006.01) C12M 1/12 (2006.01) C12M 3/06 (2006.01)
Patent
CA 1275273
IMPROVED HOLLOW FIBER CELL CULTURE DEVICE AND METHOD OF OPERATION ABSTRACT OF THE DISCLOSURE An improved cell culturing device includes a hollow fiber cartridge having a shell and a plurality of capillaries extending through the shell with at least some of the capillaries having selectively permeable walls. A call culturing space is located between the shell and capillaries. The improvement includes a chamber containing a second medium supply fluidly connected to the cell culturing space. pressurizing system pressurizes the medium within the chamber to a level higher than the level of medium flowing through the lumen of the capillaries. A valving mechanism alternatively and selectively restricts flow of medium between the chamber and the cell culturing space through first and second conduits such that circulation is effected in the cell culturing space.
485518
Cracauer Ray F.
Gruenberg Micheal L.
Walker Robert D.
Cracauer Ray F.
Endotronics Inc.
Gruenberg Micheal L.
Marks & Clerk
Walker Robert D.
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