Homo-doubly labeled compositions for the detection of enzyme...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/37 (2006.01) A61K 38/00 (2006.01) C07D 239/00 (2006.01) C07H 5/04 (2006.01) C07H 21/04 (2006.01) C07K 7/00 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12Q 1/34 (2006.01) C12Q 1/44 (2006.01) C12Q 1/48 (2006.01) G01N 21/76 (2006.01) G01N 33/00 (2006.01) G01N 33/53 (2006.01) G01N 33/542 (2006.01)

Patent

CA 2432973

The present invention provides for novel reagents whose fluorescence or absorption spectra change upon cleavage or a change in conformation of a backbone. The reagents comprise a backbone (e.g. nucleic acid,polypeptide, etc.) joining two chromophores (e.g. fluorophores) of the same species whereby the chromophores form an H-dimer resulting in quenching of the fluorescence of the fluorophores or a change in absorption spectra of the chromophores. When the backbone is cleaved or changes conformation, the chromophores are separated, no longer forming an H-type dimer, and are de-quenched thereby providing a detectable signal. The use of a single chromophore rather than an "acceptor-donor" fluorescence resonance energy transfer system offers synthesis and performance advantages.

La présente invention concerne de nouveaux réactifs dont les spectres de fluorescence ou d'absorption changent lors d'un clivage ou d'un changement de conformation d'un squelette. Les réactifs comprennent un squelette (par exemple un acide nucléique, un polypeptide, etc.) reliant deux chromophores (par exemple des fluorophores) de la même espèce de manière que les chromophores forment un dimère H ayant pour effet d'éteindre la fluorescence des fluorophores ou un changement dans les spectres d'absorption des chromophores. Lorsque le squelette est clivé ou qu'il change de conformation, les chromophores sont séparés, ne formant plus de dimère de type H, et ils ne sont plus éteints, émettant ainsi un signal détectable. L'utilisation d'un seul chromophore à la place d'un système de transfert d'énergie par résonance de fluorescence "accepteur-donneur" offre des avantages de synthèse et de performances.

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