Homocysteine desulphurase from the protozoan trichomonas...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/60 (2006.01) A61K 38/51 (2006.01) C07K 16/40 (2006.01) C12N 9/88 (2006.01) C12N 15/62 (2006.01) C12P 13/12 (2006.01) C12Q 1/527 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2264189

The present invention relates to an assay for determining homocysteine, cysteine, O-acetyl-L-serine and/or methionine levels in a biological sample using a enzyme which catalyses the degradation of homocysteine, cysteine, O- acetyl-L-serine and/or methionine, the enzyme being more particularly homocysteine desulphurase, a polynucleotide fragment encoding protozoan homocysteine desulphurase, a recombinant vector comprising such a polynucleotide fragment, transformed cells, the protozoan homocysteine desulphurase polypeptide, and pharmaceutical compositions comprising recombinant homocysteine desulphurase for use in medicine or veterinary medicine.

La présente invention se rapporte à un procédé de dosage visant à déterminer les teneurs en homocystéine, en cystéine, en O-acétyl-L-sérine et/ou en méthionine dans un prélèvement biologique. Ledit procédé implique l'utilisation d'un enzyme qui catalyse la dégradation de l'homocystéine, de la cystéine, de l'O-acétyl-L-sérine et/ou de la méthionine, ledit enzyme étant plus particulièrement une désulfurase d'homocystéine, un fragment de polynucléotide codant la désulfurase d'homocystéine protozoaire, un vecteur recombiné contenant un tel fragment de polynucléotide, des cellules transformées, le polypeptide de désulfurase d'homocystéine protozoaire. L'invention se rapporte également à des compositions pharmaceutiques contenant la désulfurase d'homocystéine de recombinaison et utilisées en médecine ou en médecine vétérinaire.

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