Homocysteine immunoassay

G - Physics – 01 – N

Patent

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G01N 33/68 (2006.01)

Patent

CA 2634899

In homocysteine immunoassays, it is known that the measured values are dispersed due to the influence of inhibiting substances present in a biological sample on the immunoreaction. The present invention provides among other things a method whereby the influence of the immunoreaction inhibiting substances is easily and efficiently suppressed or eliminated, and also provides a kit used for such a method. The influence of the immunoreaction inhibiting substances is suppressed or eliminated when the homocysteine contained in biological samples is reacted with an antibody in the presence of extrinsic polyanion.

Il est connu que, dans les dosages immunologiques d'homocystéine, les valeurs mesurées sont dispersées en raison de l'influence des substances d'inhibition présentes dans un échantillon biologique sur l'immunoréaction. L'invention concerne notamment un procédé selon lequel l'influence des substances d'inhibition de l'immunoréaction est supprimée ou éliminée de manière aisée et efficace ; l'invention porte également sur un kit utilisé pour la mise en oeuvre dudit procédé. L'influence des substances d'inhibition de l'immunoréaction est supprimée ou éliminée quand l'homocystéine contenue dans les échantillons biologiques est mise en réaction avec un anticorps en présence de polyanion extrinsèque.

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