Homogeneous amplification and detection of nucleic acids

Details Homogeneous amplification and detection of nucleic acids Homogeneous amplification and detection of nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2239683

The present invention relates to a method for detecting or amplifying and detecting a target polynucleotide sequence. The method comprises providing in combination (i) a medium suspected of containing the target polynucleotide sequence, (ii) all reagents required for conducting an amplification of the target polynucleotide sequence when amplification is desired, and (iii) two oligonucleotide probes capable of binding to a single strand of the product of the amplification. At least one of the probes has two sequences that either (i) are non-contiguous and bind to contiguous or non-contiguous sites on the single strand or (ii) can bind to non-contiguous sites on the single strand. Each probe may contain a label. The combination is subjected to conditions for amplifying the target polynucleotide sequence. Next, the combination is subjected to conditions under which both of the probes hybridize to one of the strands to form a termolecular complex, which is detected by means of the label.

L'invention concerne une méthode de détection ou d'amplification et de détection d'une séquence de polynucléotides cible. La méthode consiste à fournir en combinaison (i) un milieu soupçonné de contenir la séquence de polynucléotides cible, (ii) tous les réactifs nécessaires pour effectuer, le cas échéant, une amplification de la séquence de polynucléotides cible et (iii) deux sondes de polynucléotides capables de lier un seul brin du produit de l'amplification. Au moins une des sondes a deux séquences soit (i) non contiguës et liées à des sites contigus ou non contigus sur le simple brin soit (ii) pouvant se lier à des sites non contigus sur le simple brin. Chaque sonde peut contenir un marqueur. La combinaison est assujettie à des conditions pour l'amplification de la séquence de polynucléotides cible. Ensuite, la combinaison est soumise à des conditions sous lesquelles les deux sondes s'hybrident avec un des brins pour former un complexe termoléculaire qui est détecté au moyen d'un marqueur.

Affiliated with

Also associated with

Rating

Homogeneous amplification and detection of nucleic acids does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Homogeneous amplification and detection of nucleic acids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Homogeneous amplification and detection of nucleic acids will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1565894