Homogeneous assay of biopolymer binding by means of multiple...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2454415

A method for homogeneously assaying biopolymer bonding includes obtaining signals from a test sample before, during and/or after the application of stimulus to the test sample and correlating the signals. The signals, whose magnitude correlate with binding affinity, can be, for example, electrical conductance and/or fluorescent intensity. The stimulus can be, for example, electric voltage and/or laser radiation. Preferably, different types of signals are measured and compared so as to enhance the reliability of the assay.

L'invention porte sur un procédé d'essai homogène de fixation d'un biopolymère consistant à obtenir des signaux d'au moins un échantillon test avant, durant et après l'application de stimuli audit échantillon test, puis à corréler les signaux. Les signaux, dont l'amplitude est en corrélation avec les affinités de fixation, peuvent par exemple consister en intensités de conductance électrique et/ou de fluorescence. Les stimuli peuvent par exemple être une tension électrique et/ou un rayonnement laser. On mesure et on compare de préférence différents types de signaux pour accroître la fiabilité de l'essai.

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