Homogeneous assay on a non-porous surface

Details Homogeneous assay on a non-porous surface Homogeneous assay on a non-porous surface

G - Physics

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150/15

G01N 33/58 (2006.01) C12Q 1/26 (2006.01) C12Q 1/34 (2006.01) G01N 33/542 (2006.01)

Patent

CA 1209889

HOMOGENEOUS AS SAY ON A NON-POROUS SURFACE ABSTRACT OF THE DISCLOSURE Enzyme channeling assay is provided employing a solid non-porous surface to which is bound a member of a specific binding pair and an enzyme. A complementary member of a specific binding pair is conjugated to second enzyme, so that the amount of second enzyme which becomes bound to said solid non-porous surface by the binding of a specific binding pair(s) is related to the amount of analyte in the liquid assay medium with which the solid surface is in contact. The first and second enzymes are related by the product of one being the substrate of the other. The turnover of the sub- strate formed by one of the enzymes results in a detectable product, which can be related to the amount of analyte in the medium.

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