Homogeneous detection method

G - Physics – 01 – N

Patent

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G01N 33/536 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2547351

The invention relates to methods for the quantitative or qualitative detection of an analyte in an assay and adequate reagents therefor, particularly a homogeneous binding test. According to the invention, an analyte-specific binding partner R1 comprises more than one specific binding point for a specific binding partner X that is associated with a component of a signal- forming system while a second analyte-specific binding partner R2 comprises more than one specific binding point for a specific binding partner Y which is also associated with a component of a signal-forming system.

L'invention concerne des procédés servant à la détection quantitative ou qualitative d'un analyte dans un échantillon, ainsi que des réactifs appropriés, notamment un test de liaison homogène. Selon l'invention, un partenaire de liaison R1 spécifique de l'analyte présente plus d'un point de liaison spécifique pour un partenaire de liaison spécifique X qui est associé à un constituant d'un système formateur de signal, et un deuxième partenaire de liaison R2 spécifique de l'analyte présente plus d'un point de liaison spécifique pour un partenaire de liaison spécifique Y qui est également associé à un constituant d'un système formateur de signal.

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