Homogeneous enzymatic assay for vitamin b6 and improvements...

Details Homogeneous enzymatic assay for vitamin b6 and improvements... Homogeneous enzymatic assay for vitamin b6 and improvements...

C - Chemistry, Metallurgy

12

Q

C12Q 1/527 (2006.01) C12N 9/88 (2006.01) C12Q 1/00 (2006.01) C12Q 1/37 (2006.01) C12Q 1/48 (2006.01) C12Q 1/52 (2006.01) G01N 21/78 (2006.01) G01N 31/00 (2006.01) G01N 33/82 (2006.01)

Patent

CA 2361077

Enzymatic methods to determine the concentration of pyridoxal 5'-phosphate (PLP) in biological fluids are described. The methods of the invention are useful to assess risk for cardiovascular disease. The assay can be a homogeneous assay using the ability of PLP to function as a co-enzyme for homocysteinase and related enzymes and measuring the products of the reaction preferably spectrophotometrically. The invention also includes improvements in sensitivity of assays for measuring hydrogen sulfide production by measuring fluorescence as opposed to absorbance of the oxidized product of H2S with N, N-dialkyl p-phenylene diamine.

La présente invention a pour objet des méthodes enzymatiques pour déterminer la concentration de 5'-phosphate de pyridoxal (PLP) dans des fluides biologiques. Lesdites méthodes sont utiles pour évaluer les risques de maladie cardio-vasculaire. L'épreuve peut être une épreuve homogène basée sur la capacité du PLP à fonctionner comme une coenzyme pour l'homocystéinase et les enzymes connexes et permettant de mesurer les produits de la réaction, de préférence par spectrophotomètrie. Ladite invention a aussi pour objet des améliorations de la sensibilité de ces épreuves pour mesurer la production d'hydrogène sulfuré par la fluorescence plutôt que par l'absorption du produit d'oxudation de H2S avec la N, N-dialkyl-p-phénylènediamine.

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