C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
167/103.1
C07K 14/565 (2006.01) A61K 38/00 (2006.01)
Patent
CA 1146468
EV 4100/14 Abstract A process for producing human fibroblast inter- feron as a homogeneous protein which process comprises the following steps in combination: A. passing an aqueous solution of human fibroblast inter- feron in an impure state through an affinity chromato- graphy column so as to absorb said interferon onto said column and thereafter eluting said interferon from said column and obtaining said interferon in selected frac- tions of said eluate in a state of enhanced purity; and B. passing the selected said interferon fractions ob- tained in Step A through one or more buffer equilibrated hydrocarbon bonded silica matrix column(s) under high pres- sure liquid chromatography conditions wherein said hy- drocarbon is selected from the group consisting of cy- clohexyl, phenyl, diphenyl, octyl, octadecyl and cyano- propyl, to absorb said interferon onto said column and thereafter eluting said interferon from said column with a gradient of an acidic aqueous buffered mixture of water miscible solvents and obtaining interferon as a single distinct peak in selected fractions of said eluate in the state of a homogeneous protein.
357224
Friesen Heinz-Jurgen
Pestka Sidney
Gowling Lafleur Henderson Llp
Hoffmann-La Roche Limited
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