Homogeneous fluoroassay methods employing fluorescent...

Details Homogeneous fluoroassay methods employing fluorescent... Homogeneous fluoroassay methods employing fluorescent...

G - Physics

01

N

167/43

G01N 33/536 (2006.01) G01N 33/533 (2006.01) G01N 33/542 (2006.01)

Patent

CA 1309016

ABSTRACT OF THE DISCLOSURE Homogeneous assays for determining quantitatively the extent of a specific binding reaction can be carried out effectively on very dilute solutions using measurements of fluorescence if a fluorescence measurement scheme that is capable of rejecting short-lived background fluorescence is employed and if the fluorescent group being measured has the following properties: a. the group being measured must be a rare earth metal chelate complex combination; b. the chelate must be water-soluble; c. the complex combination must also be stable in extremely dilute aqueous solutions, that is, the measured chelate must have at least one ligand having a metal-to-ligand binding constant of at least about 1013M-1 or greater and it must have a fluorescent emission that is long-lived compared to the longest decay lifetime of ambient substances and have a half life of from 0.01 to 50 msec.

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