Homogeneous immunoassay using enzyme inhibitors

Details Homogeneous immunoassay using enzyme inhibitors Homogeneous immunoassay using enzyme inhibitors

G - Physics

01

N

G01N 33/535 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2076291

HOMOGENEOUS IMMUNOASSAYS USING ENZYME INHIBITORS ABSTRACT OF THE DISCLOSURE A method for determining the presence of an analyte which is a specific binding pair member in a sample suspected of containing the analyte is described. The method involves: bringing together in an aqueous medium, the sample, an enzyme bound to a first specific binding pair member and an inhibitor for the enzyme bound to a second specific binding pair member wherein each specific binding pair member is capable of binding to the analyte or to a specific binding pair member complementary to the analyte; analyzing the medium for the activity of said enzyme; and relating the activity to the amount of analyte present in the medium. Compositions of matter and kits are also described. MIM: 10643 27370--FF

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