Homogeneous method for assay of double-stranded nucleic...

Details Homogeneous method for assay of double-stranded nucleic... Homogeneous method for assay of double-stranded nucleic...

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2148140

A highly sensitive homogeneous assay allows for quantitative detection of amplified nucleic acids. This detection is achieved during or after amplification with a high affinity fluorescent dye which is from the class of unsymmetrical cyanine dyes having at least two positive charges and a binding constant (Kb) within the range of from about 1 x 104 to about 5 x 105 (molar-1). The reagents used for the assay can be contained in a kit designed for amplification such as by polymerase chain reaction.

Essai homogène hautement sensible permettant la détection quantitative d'acides nucléiques amplifiés. La détection a lieu pendant ou après l'amplification, à l'aide d'un colorant fluorescent à affinité élevée provenant de la classe des colorants cyaniques asymétriques, possédant au moins deux charges positives et une constante d'association (Kb) située entre environ 1 x 10 4 et environ 5 x 10 5 (mol-1). Les réactifs utilisés dans cet essai peuvent être contenus dans une trousse conçue pour l'amplification, par exemple par réaction en chaîne de la polymérase.

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