Homogeneous multiplex screening assays and kits

Details Homogeneous multiplex screening assays and kits Homogeneous multiplex screening assays and kits

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2524080

Highly multiplexed homogeneous in vitro screening assays for numerous possible nucleic acid targets, any of which might be present in a sample, utilize fluorescent hybridization probes that are combinatorially coded from a panel of fluorophores by subdividing each probe into portions and differently labeling each portion such that, when portions are combined, each probe has a unique code. The assays may include target amplification and real-time detection. Probe sets and kits containing additional assay reagents may be used to perform the screening assays.

L'invention a trait à des dosages biologiques in vitro homogènes hautement multiplexés, qui permettent de rechercher un grand nombre d'acides nucléiques cibles potentiels, n'importe lequel de ces derniers pouvant être présent dans un échantillon. Lesdits dosages font appel à des sondes d'hybridation fluorescentes, que l'on code de manière combinatoire à partir d'un groupe de fluorophores, en divisant chaque sonde en plusieurs parties et en marquant différemment chaque partie de manière que, lorsque les parties sont combinées, chaque sonde possède un code unique. Les dosages peuvent comprendre l'amplification et la détection en temps réel de cibles. Des ensembles et des kits de sondage contenant des réactifs de dosage supplémentaires peuvent être utilisés pour la réalisation des dosages biologiques.

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