Homogeneous nucleic acid hybridization diagnostics by...

Details Homogeneous nucleic acid hybridization diagnostics by... Homogeneous nucleic acid hybridization diagnostics by...

G - Physics

01

N

150/1, 150/8.5

G01N 33/58 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1190838

ABSTRACT A homogeneous light-emitting hybridization assay is disclosed wherein light-labeled first and second single-stranded reagent segments are hybridized with a complimentary target single- stranded polynucleotide from a physiological sample such that non-radiative energy transfer occurs between the light labels of the two reagent segments. At least one of the light labels is of the absorber/emitter type such that energy absorbed from the other light label is re-emitted as a different wavelength. Such secondary emissions can only occur if hybridization has taken place and hence the presence of the target polynucleotide is related to the amount of secondary light emitted.

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