Homogeneous specific binding assay device and method

Details Homogeneous specific binding assay device and method Homogeneous specific binding assay device and method

G - Physics

01

N

167/45, 150/5, 7

G01N 33/58 (2006.01) G01N 33/542 (2006.01)

Patent

CA 1183450

ABSTRACT OF THE DISCLOSURE A homogeneous specific binding assay device, a method for its preparation, and a method for its use in determining a ligand, such as antigen, hapten, or antibody, in, or the ligand binding capacity of, a liquid sample. The test device comprises a solid carrier member, such as a fibrous web matrix, e.g. paper, or a polymeric film or gel, incorporated with reagents for a homogeneous specific binding assay system which produces a detectable response, usually an electromagnetic radiation signal, that is a function of the presence or amount of the ligand in or the ligand binding capacity of the sample. Useful homogeneous specific binding assay systems include those involving enzyme substrate labels, enzyme prosthetic group labels, and enzyme labels. The detectable response preferably is a luminescent, fluorescent, spectrophotometric, or colorimetric response, which is measurable by visual observation or instrument means.

381675

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