Homogenous assay system

Details Homogenous assay system Homogenous assay system

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2088683

A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.

Un procédé de détection d'un acide nucléique cible à l'aide d'oligonucléotides marqués utilise l'activité nucléase 5' à 3' d'une polymérase d'acide nucléique pour couper un oligonucléotide marqué et circularisé de duplex hybridés, et pour libérer des fragments nucléotidiques marqués pour la détection. Ce procédé s'intègre facilement à un dosage d'amplification de la réaction en chaîne de la polymérase.

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