Homogenous radioimmunoassay with second addition of serum...

G - Physics – 01 – N

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G01N 23/00 (2006.01) G01N 33/48 (2006.01)

Patent

CA 1113390

HOMOGENOUS RADIOIMMUNOASSAY WITH SECOND ADDITION OF SERUM SAMPLE ABSTRACT OF THE DISCLOSURE Disclosed is an in vitro method of determining the thyroid function in blood serum. The method com- prises mixing a blood serum sample of a patient with a solution which contains radioactive thyroxine and with a compound which liberates thyroxine from thyroxine- binding globulin, buffering the mixture with a buffer- ing agent, adding another sample of the patient's blood serum in a quantity which is smaller than that of the initial blood serum sample, adding an antiserum con- taining antibodies for an immunoreaction with thyroxine, separating the non-bound thyroxine from the thyroxine bound to antibodies, and determining their relative quantities by measuring the radioactivity.

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