Homologous recombination antibody expression system for...

Details Homologous recombination antibody expression system for... Homologous recombination antibody expression system for...

C - Chemistry, Metallurgy

12

N

C12N 15/90 (2006.01) C07K 16/00 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2177959

A specific locus in the genome of a murine host cell is identified which causes high levels of recombinant gene expression following stable integration, via homologous recombination, of the recombinant gene into the specific chromosomal locus. The selection of a favorable genome locus for the insertion and expression of a recombinant gene is disclosed, as are DNA vectors and host cells.

On a identifié un locus spécifique dans le génome d'une cellule hôte murine occasionnant de hauts niveaux d'expression de gènes recombinés après une intégration stable, par recombinaison homologue, du gène recombiné dans le locus chromosomique spécifique. L'invention se rapporte également au choix d'un locus génomique favorable destiné à l'insertion et à l'expression d'un gène recombiné, ainsi qu'aux vecteurs d'ADN et aux cellules hôtes correspondants.

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