Homologous recombination for universal donor cells and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/23 (2006.01) A01K 67/027 (2006.01) A61K 49/00 (2006.01) C07K 14/74 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2180019

Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta.2-microglobulin gene is inactivated for reducing or eliminating the expression of functional Class I MHC antigens. The resulting cells may be used as universal donor cells. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies. Methods for homologous recombination in non-transformed mammalian somatic cells are also described.

On utilise des techniques de recombinaison homologue pour inactiver des gènes, notamment des gènes associés avec des antigènes MHC. En particulier, on inactive le gène de .beta.¿2?-microglobuline afin de réduire ou d'éliminer l'expression d'antigènes fonctionnels MHC de classe I. On peut utiliser les cellules résultantes comme cellules donneuses universelles. On peut modifier, en outre, des cellules souches embryonnaires par recombinaison homologue afin de les utiliser pour produire des hôtes mammaliens chimériques ou transgéniques, qui peuvent être utilisés comme donneurs universels d'organes ou comme modèles pour des thérapies à base de médicaments ou de transplantations. On décrit également des procédés de recombinaison homologue dans des cellules somatiques mammaliennes non transformées.

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