C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/90 (2006.01) C12N 15/85 (2006.01)
Patent
CA 2263958
The present invention relates to a method for modifying the genome of eukaryotic cells by homologous recombination using DNA sequences which substantially differ from the target locus with respect to the nucleotide sequence (0.1 to 30 % divergence) in the region where recombination takes place. Homologous recombination between diverged sequences is achieved by genetic or transitory inactivation of the cell's mismatch repair system.
La présente invention se rapporte à un procédé de modification du génome de cellules eucaryotes par recombinaison homologue de séquences d'ADN qui diffèrent sensiblement du locus cible par rapport à la séquence nucléotidique (0,1 à 30 % de divergence) dans la région où se produit la recombinaison. On parvient à une recombinaison homologue entre des séquences divergentes par inactivation génétique ou transitoire du système de réparation des mésappariements des cellules.
de Wind Niels
Dekker-Vlaar Helena Maria Johanna
Te Riele Henricus Petrus Joseph
Mixis France
Robic
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