Horseshoe crab amebocyte lysate factor g subunit a and dna...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) C07K 14/435 (2006.01) C12N 9/24 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2143621

This invention relates to a DNA shown by SEQ ID No. 1 and an amino acid sequence coded by said DNA. This invention also relates to a DNA and an amino acid sequence of subunit a of (1.fwdarw.3)-.beta.-D-glucan sensitive factor derived from amebocytes of horseshoe crab and have potent affinity to (1.fwdarw.3)-.beta.-D-glucan in cell walls of fungi. Therefore, the invention is useful for the diagnosis of fungal diseases and as antimicrobial or eradicating agent of fungi in combination with an antifungal agent.

ADN représenté par SEQ ID NO:1, et séquence d'acides aminés codée par cet ADN. L'ADN et la séquence sont ceux de la sous-unité a &cir& _ d'un facteur sensible à la (1}3)- beta -D-glucanne originaire des amoebocytes de Limulus, et ils présentent une grande affinité pour la (1}3)- beta -D-glucanne dans la paroi cellulaire d'un champignon. Par conséquent, ils sont utilisables dans le diagnostic clinique de la mycose, comme agent antibactérien utilisé en association avec un agent antifongique et dans l'élimination des champignons.

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