Host-derived signals for inducing isoprenoid gene expression...

C - Chemistry – Metallurgy – 12 – P

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C12P 1/00 (2006.01) A01N 61/00 (2006.01) A01N 65/00 (2006.01) C12N 9/10 (2006.01)

Patent

CA 2251117

Disclosed is a method for preparing a composition that is capable of activating the expression of a gene involved in the synthesis of an isoprenoid, the method involving: (a) contacting a plant cell with an elicitor under conditions that allow an elicitor-induced release of a compound that activates the synthesis of an isoprenoid; and (b) recovering a composition including the compound, wherein the compound is diffusible and has a molecular weight less than or equal to 10,000 daltons. Also disclosed is a substantially pure elicitor-induced composition, the composition being capable of activating a gene involved in the synthesis of a plant isoprenoid.

La présente invention concerne un procédé de préparation d'une composition capable d'activer l'expression d'un gène intervenant dans la synthèse d'un isoprénoïde. Ce procédé consiste: (a) à mettre en contact une cellule végétale avec un éliciteur sous des conditions qui permettent, par induction par l'éliciteur, la libération d'un composé qui active la synthèse d'un isoprénoïde; et (b) à récupérer une composition incluant le composé, lequel composé est capable de se diffuser et présente une masse moléculaire inférieure ou égale à 10.000 Daltons. L'invention concerne également une composition sensiblement pure induite par l'éliciteur, laquelle composition est capable d'activer l'expression d'un gène intervenant dans la synthèse d'un isoprénoïde végétal.

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